University of Twente Student Theses
Optimization of a Gradient Generating Microfluidic System for Quantification of the Minimal Metabolic Requirements of Mesenchymal Stem Cells in Anoxia
Schreurs, Y.WE. (2021) Optimization of a Gradient Generating Microfluidic System for Quantification of the Minimal Metabolic Requirements of Mesenchymal Stem Cells in Anoxia.
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| Abstract: | Tissue regeneration is an up and coming field within the life sciences with drastically increasing investigations regarding repair or replacement of damaged or missing tissue. One of the main challenges within tissue engineering is the unification of the foreign material, incorporated with hMSCs, with the donor site. The origin of this problem is the lack of vascularization at the donor site which results in an anoxic core in the implant, which turns necrotic. This project aims to optimise a gradient generating microfluidic system. This system will eventually be used to obtain data on the minimal metabolic requirements of hMSCs in anoxia. With this data hMSCs incorporated in implants can be supplied with sufficient metabolites to survive the period in which it is not yet connected to the donor site. This dynamic system is optimised with the use of 3T3 cells by tuning several variables within the set up, such as the chip design, tubing, and flow rate. Biological variables within the system have been optimised with hMSCs in a static environment and tested in the dynamic set up. These variables include but are not limited to; coating, donor, and medium. The effect on the hMSCs is assessed with live/dead assays, PrestoBlue assays, and visual observations. A stable gradient generating microfluidic system is optimised, in which several concentrations of metabolites can be tested for their effect on hMSCs in anoxia. An important variable within the system is the choice of hMSC donor, on which the functionality of the system depends. With this system a data set can be constructed on the minimal metabolic requirements for hMSCs in anoxia and provide a stepping stone for future work with hMSCs in tissue engineered constructs regarding metabolism. |
| Item Type: | Essay (Master) |
| Faculty: | TNW: Science and Technology |
| Programme: | Biomedical Engineering MSc (66226) |
| Link to this item: | https://purl.utwente.nl/essays/88101 |
| Export this item as: | BibTeX EndNote HTML Citation Reference Manager |
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